Next Gen Sequencing Scopes

Sequence-by-synthesis through Illumina Sequencing platform

Elim strives to provide you with the most advanced cutting-edge technologies for your needs in genomics. Currently, Elim uses the industry-leading Illumina sequencing platform (Genome Analyzer IIx and HiSeq2000 sequencers) for your Next Gen Sequencing.

How Next Gen Sequencing works:

Your sample libraries are prepared and annealed to a flow cell. Each flow cell has 8 lanes, so you have the opportunity to run different templates for each lane. The prepared libraries are amplified into millions of clonal clusters, which are then sequenced one base at a time, in parallel, for each of the millions of clusters. The base calling (sequencing data gathering) steps include incorporation of fluorophore-labeled nucleotides, fluorescence capture of the incorporated nucleotide, and cleavage of the fluorescence molecule. Each of these cycles are run 36, 50, 75, or 100 times, depending on your experimental requirements. There is also the option to run either single read (only one end of the template clonal clusters will be sequenced) or paired-end read (both ends of the template clonal clusters will be sequenced). A Real Time Analysis program actively interprets the raw data to determine each base being incorporated as the sequence-by-synthesis occurs. Benefits of Elim's Next Gen Sequencing include:

    • Short wait time and fast turnaround
    • Extremely high throughput
    • Scalable: Up to eight samples can be loaded onto the flow cell       simultaneously
    • High accuracy
    • Cost effective
    • Library construction services
    • Bioinformatics services

The scope of Elim's Next Gen Sequencing Services includes many cutting-edge applications, such as:

De novo Sequencing
Assemble contigs from millions of reads without a reference sequence.

Whole-Genome Resequencing
Discovery of genome-wide changes—copy number variations, chromosomal rearrangements (deletions, insertions, translocations, etc.), and single-nucleotide variations.

Targeted Sequencing (e.g., Exome Sequencing)
Isolation of specific targeted regions of the genome (all coding exons, immunoglobulin switching regions, regions identified by association studies) by long PCR or hybridization to oligonucleotide synthesized on arrays.

Whole-Transcriptome Profiling (e.g., mRNAseq, Tag Profiling)
Sequencing of random-primed cDNA libraries from RNA fractions (nuclear, cytoplasmic, polyA, capped, or small RNA) provides a high-resolution map of all RNA species. Sequencing of tags created by restriction digestion of cDNA generates gene expression profiles with an absolute count (from one to a few million) of the RNA in the sample.

Protein-DNA or Protein-RNA Interactions (e.g., ChIP sequencing)
Discovery of functional transcription factor binding sites across the whole genome and determination of patterns of DNA occupancy by nucleosomes, polymerases, etc. via immunoprecipitation of proteins bound to nucleic acids and the subsequent sequencing of the associated DNA or RNA (ChIP sequencing); selective isolation of DNA via enzyme trapping methods such as trapping of methyltransferases by DNA labeled with aza-nucleotides.

Determination of DNA methylation variation across the whole genome via bisulfite sequencing or sequencing of fragments generated by methylation restriction polymorphisms, purification of methylated fragments by antibody affinity, or methyltransferase trapping.

And Much More...
Contact us so that we can assist in designing and running your Next Gen Sequencing project! We look forward to working with you!
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