Elim Biopharm

Whole Plasmid Sequencing

$15/plasmid, Overnight Turn-Around
Time, Convenient Local Sample Pickup

Whole Plasmid Sequencing

Speed, Simplicity, Cost Advantage

Elim Whole Plasmid Sequencing service is a cost-effective, fast, and convenient way to verify your whole plasmid sequences. We obtain a high-accuracy consensus of your plasmid and deliver the sequencing results to you overnight.

Overnight turn-around time

  • Submit samples via sample drop box at one of our many convenient pick-up locations around the Bay Area (free local sample pick up) or ship it to our facilities. Receive sequencing results for the plasmids the next business day after your samples arrive.

Cost-effective

  • $15/sample, sequence plasmids up to 25 kb

No primers or PCR amplification needed

  • Whole Plasmid Sequencing does not require primers, and uses long-reads to produce a highly accurate consensus sequence of your plasmids, with each base covered multiple times
  • Whole plasmid sequencing works great for GC-rich regions and repetitive regions

What you will receive from our

Whole Plasmid Sequencing Services

Fastq (quality scores) and FASTA (consensus/assembly) files for the sequenced plasmid

Alignment file for the plasmid compared to reference, if you provide a reference sequence for the plasmid during the ordering process

Annotated plasmid map of the regions of interest

Whole Plasmid Sequencing

Submission Guidelines

Plasmid DNA

Submit at least 10 ul of sample at >= 50 ng/ul. If you have lower concentration samples, please submit as much as possible.

Submission Tips

  • Submit samples in water or 10mM Tris. If possible, please do not use buffers containing EDTA.
  • Submit plasmid samples in 200ul strip tubes, or 96-well plates. Do not use 1.5ml tubes, or other tube formats. Please cap/seal all samples well, to avoid leaking during transport or possible contamination.

Plasmid Purity

As measured by OD: aim for OD 260/280 of 1.8-2.0, and OD 260/230 of 2.0-2.2.

Labeling

If using strip tubes, label samples with your initials on the first sample, and sample # on the tube for all tubes. If using plate, please label your order number and initials on the plate. If you are submitting plates, do not skip wells between samples, and start your samples at A1.

Ordering Process

1

Place Order

Once you have created an account, please log in and place an order online for whole plasmid sequencing.

2

Send Plasmid Samples

Print the order confirmation and place in a bag with your samples. Seal your plasmid samples tightly to prevent any spillage. Please submit in only strip tubes or PCR plates.

We offer free sample pick up for local customers in the San Francisco Bay Area. For customers outside of the Bay Area, please ship to our address:
Elim Biopharmaceuticals, Inc.
Attn: Whole Plasmid Sequencing
25495 Whitesell Street,
Hayward, CA 94545, USA

3

Check Results

You will be notified by email when the results are available online. The results are available overnight (the next business day), after we receive the samples.

Please log-in to our website and download the results.